The use of coherent light in conventional holographic phasemicroscopy (HPM) poses three major drawbacks: poor spatial resolution,weak depth sectioning, and fixed pattern noise due to unwanted diffraction.Here, we report a technique which can overcome these drawbacks, butmaintains the advantage of phase microscopy - high contrast live cellimaging and 3D imaging. A speckle beam of a complex spatial pattern isused for illumination to reduce fixed pattern noise and to improve opticalsectioning capability. By recording of the electric field of speckle, wedemonstrate high contrast 3D live cell imaging without the need for axialscanning - neither objective lens nor sample stage. This technique has greatpotential in studying biological samples with improved sensitivity,resolution and optical sectioning capability.
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